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American Society of Microbiology ABMM Sample Questions:

1. During a community outbreak of influenza, a public health laboratory performs rapid antigen testing on nasopharyngeal swabs. A test for influenza A is positive, but the influenza B test is negative. Subsequently, RT-PCR is performed on the same sample, and it is negative for both influenza A and B viral RNA. The MOST likely explanation for this discordant result is:

A) The rapid antigen test has low sensitivity, resulting in a false positive.
B) The RT-PCR assay has a high limit of detection, missing a low viral load.
C) The rapid antigen test yielded a false positive result, and the RT-PCR was a true negative.
D) The patient was infected with a novel influenza strain not detected by either assay.

2. A clinical microbiology laboratory is preparing a Gram stain of a mixed bacterial culture containing both Gram-positive and Gram-negative organisms. The correct order of reagents used in the Gram staining procedure is:

A) Iodine, crystal violet, decolorizer, safranin
B) Decolorizer, crystal violet, iodine, safranin
C) Safranin, crystal violet, decolorizer, iodine
D) Crystal violet, iodine, decolorizer, safranin

3. Which of the following organisms is an obligate intracellular bacterium transmitted by arthropod vectors and is a common cause of Rocky Mountain Spotted Fever?

A) Chlamydia trachomatis
B) Coxiella burnetii
C) Borrelia burgdorferi
D) Rickettsia rickettsii

4. A research laboratory is studying the pathogenesis of respiratory syncytial virus (RSV) infection in infants.
They find that a specific viral surface glycoprotein is primarily responsible for attachment to host cells. This glycoprotein is:

A) Matrix protein (M)
B) Fusion protein (F)
C) Neuraminidase (N)
D) Hemagglutinin (H)

5. A research laboratory is investigating the mechanisms of antigenic variation in Trypanosoma brucei, the causative agent of African trypanosomiasis. The parasite evades the host immune response by periodically changing its surface coat protein. This process primarily involves:

A) High-frequency point mutations in the gene encoding the variant surface glycoprotein (VSG).
B) Segmental gene conversion events involving a large repertoire of silent VSG genes.
C) Recombination between different VSG gene loci on minichromosomes.
D) RNA interference-mediated silencing of expressed VSG genes followed by activation of a new VSG gene.

Solutions: